Retarding Field Integrated Fluorescence and Electron ...
Ultra-thin (80 nm) sections were cut and collected on indium tin oxide (ITO) glass (Optics Balzers). The sections were incubated with guinea pig anti-insulin antibody (Invitrogen lot # SE2381176) followed by a biotinylated donkey anti-guinea pig secondary antibody (Jackson-IR lot # 137834) and finally a streptavidin-conjugated Alexa 594 ...
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